Assembly of the Nicotinic Acetylcholine Receptor
نویسندگان
چکیده
منابع مشابه
Determinants Responsible for Assembly of the Nicotinic Acetylcholine Receptor
The assembly of the four homologous, but distinct, a , b , g , and d subunits of the nicotinic acetylcholine receptor (nAChR) into the pentamer a 2 bgd presents a unique opportunity to delineate the individual amino acid side chains that contribute to the assembly process, and to examine the pathway responsible for subunit assembly and expression at the cell surface. It is well established that...
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Through specific intersubunit contacts, the four subunits of the nicotinic acetylcholine receptor assemble into an alpha 2 beta gamma delta pentamer. The specificity of subunit association leads to formation of proper ligand binding sites and to transport of assembled pentamers to the cell surface. To identify determinants of subunit association, we constructed chimeric subunits, transfected th...
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The structural elements required for normal maturation and assembly of the nicotinic acetylcholine receptor alpha subunit were investigated by expression of mutated subunits in transfected fibroblasts. Normally, the wild-type alpha subunit acquires high affinity alpha bungarotoxin binding in a time-dependent manner; however, mutation of the 128 and/or 142 cysteines to either serine or alanine, ...
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We report here the biologically active conformation of acetylcholine when bound to the high-affinity state of the receptor from Torpedo californica. The acetylcholine conformation was determined in the free and bound states by proton NMR two-dimensional nuclear Overhauser effects. In agreement with x-ray crystallographic data, acetylcholine in solution has an extended conformation with an avera...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.44.27575